Our microbial cell line development services aim to develop high producing clones. We include adapted product quality analysis into our selection process to ensure that not only productivity but also critical product attributes targets are met.
Our team has extensive experience using a diversity of E. coli established systems for the production of challenging proteins. We offer optimised IP-free vectors that are designed for improved protein expression and stability and have successfully developed processes with a diversity of expression strategies: cytoplasmic, periplasmic, soluble and insoluble (inclusion bodies).
For secreted proteins, we will most often opt for expression in P. pastoris (now renamed Komagataella phaffii) which is an established, FDA approved and highly competitive expression host. Our team has successfully produced a diversity of proteins (enzymes, cytokines, antibody-derived fragments…) using the methanol-inducible AOX promoter or the constitutive GAP promoter.
Our approach consists in screening a complete set of parameters in order to select the optimal strain and expression conditions:
The key when developing a P. pastoris manufacturing strain is the capacity to screen a large number of clones to select the hyper producing ones. To that purpose, GTP has developed a high-throughput microscale cultivation process.
A classical P. pastoris strain development project includes the following steps: