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Antibody engineering & custom production

GTP offers antibody engineering and custom production services for therapeutic antibody discovery and development projects. These services range from antibody engineering & humanisation to the production of material of your lead antibody or antibody variants for preclinical in vitro and in vivo assays.

Move your monoclonal antibody candidate from the discovery to the preclinical stage

Extensive expertise in antibody engineering and mAbs production

GTP has managed hundreds of antibody engineering and production projects. Our services cover the production of classical monoclonal antibodies as well as the expression and purification of modified antibodies such as antibody fusion proteins, bispecific antibodies, and antibody fragments (ScFv, VHH, Fab, minibodies…). Our antibody engineering expertise includes:

Antibody humanisation
  • Hybridoma sequencing
  • Isotype, subtype and/or species switching
  • Reformatting
  • Humanisation
  • Bispecific antibodies design (knobs-into-holes)

A unique antibody humanization process

Antibody humanisation turns murine antibodies into humanized mAbs, a crucial step in the development of your therapeutic antibody candidate. For this step, GTP partners with a consultant who has over 25 years of experience and a successful track record in antibody engineering and development. Our combined expertise has resulted in a unique humanisation process in two rounds, with a combination of CDR grafting, in silico molecular modelling and framework optimisation. The objective of this process is to deliver an antibody sequence that not only complies with WHO guidelines for humanized antibodies, but also improves your antibody's manufacturability and affinity.

All the material you need for your in vitro and in vivo studies

GTP provides all the antibody material you need to validate biological activity, screen and optimize antibody humanisation variants or perform preclinical trials in animal models.

In order to deliver this material in short turnaround times, we use an efficient transient transfection platform for the production of antibody quantities, ranging from micrograms to grams. Thanks to optimized expression conditions, we routinely obtain high expression yields for a variety of human and mouse antibodies. We can produce up to a hundred antibodies in parallel for screening purposes in either  CHO or HEK cells, depending on your requirements.

Depending on your project's requirements, we also develop stably transfected pools, which are easier to scale up compared to transient transfection.

 

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