Analytical methods

GTP offers an extensive range of analytical methods to support your recombinant protein project. Enzymes, antigens, antibodies and peptides have no secrets for us: we have worked on over 800 diverse recombinant protein projects to vouch for our expertise. Our analytical services are customised to meet your recombinant protein production project needs, from product characterisation to the development of custom analytical packages.

Reliable and comprehensive analytical services to support all your recombinant protein production projects

Expert analytic methods to help your protein project move forward

Whether your recombinant protein or antibody will be used in research, during preclinical in vivo experiments or become the reagent in a marketed product, we provide reliable and relevant analytical packages. GTP has over 15 years of experience in recombinant protein projects at every stage of development. We master an extensive range of analytical methods to expertly support your project.

Our comprehensive set of methods includes in-process testing, product characterization, characterization of critical attributes and formulation as well as stability studies. To fit your project's specific needs, we draw from our experience and select the most appropriate analytical techniques and methods.

Our service include analytical methods transfer, development, qualification and routine testing to help your recombinant protein project move forward.

Electrophoretic techniques

  • Capillary electrophoresis
  • IEF
  • Western-blot


  • Size exclusion
  • Reverse phase
  • Ion exchange
  • Affinity

Process residuals

  • HCP determination by ELISA
  • Endotoxins quantification by LAL
  • DNA quantification by qPCR
  • Microbiological contamination (Bioburden)

Potency assays

  • Enzymatic activity
  • Binding assays via Elisa and BioLayer Interferometry (BLItz®)

Mass spectrometry

  • Intact mass
  • Peptide mapping
  • Post translational modifications
  • Glycan profile

Biophysical characterisation

  • DLS
  • MALS
  • Thermal shift assay (Thermofluor®)


Most analyses are performed in-house. For mass spectrometry, we have an established partnership with Proteodynamics. They are experts in glycosylation, glycomics and in the characterisation of proteins and peptides by mass spectrometry and analytical chromatography.

Analytical methods 2