Based on our experience with over hundreds of protein and antibody projects, we know how crucial the optimized design of genes and constructs is to the expression of challenging recombinant proteins and antibodies.
Our scientists have a strong background in molecular biology on which we have drawn over time to develop tricks and tools to increase expression.
We have developed our own gene optimisation tool (see our blog article “How to make gene optimisation for expression in Escherichia coli a piece of cake”) which takes into account parameters that are usually left aside by gene synthesis companies (such as mRNA secondary structure in the translational initiation and slippage-prone sequences). We use this tool on every project that requires synthesis.
We offer a set of commercial vectors as well as our own customised vectors to fit all classical expression systems.
For E. coli for example, we have developed four IP-free vectors with different features, all designed to improve protein expression and stability. For projects with an industrial application, we will most often suggest the use of these vectors.
For projects that require specific construct design, we are pleased to offer consultancy services to help you develop the most efficient system for your challenging protein.