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Recombinant protein production in E. coli
Tailored services for the production of your recombinant protein in Escherichia coli
Drawing on our experience on hundreds of projects, our team offers expert guidance to define a relevant strategy for an efficient expression of your protein in E. coli.
Unique expertise in E. coli protein expression
The team at GTP has successfully produced hundreds of challenging proteins and developed efficient processes for cytoplasmic, periplasmic and soluble or insoluble expression of proteins in E. coli.
Whether your project aims to develop a therapeutic or vaccine protein, to obtain material for research studies or to develop a diagnostic tool, we always consider E. coli to be an excellent first choice – except when expressing complex proteins that require post-translational modifications.
Integrated services for protein production in Escherichia coli
We have the expertise and capabilities to manage complete E. coli production projects from gene synthesis through to milligram to decagram-scale batch production.
Our custom services include:
- E. coli strain development, including expression vector design & construction
- Yield optimisation in small-scale feasibility studies for soluble / insoluble (inclusion bodies), periplasmic/cytoplasmic expressions
- Screening and optimisation of critical parameters for solubilisation and refolding.
- Development of batch and fed-batch fermentations
- Development/optimisation of the purification process
Unique tools for optimised expression in E. coli
Our experts have developed advanced tools for enhanced expression of your protein in E. coli.
When gene synthesis is required, we use our own gene optimisation program. This tool takes into account parameters that we consider critical for improved protein expression in E. coli that are usually left aside by gene synthesis companies (see our blog article “How to make gene optimisation for expression in Escherichia coli a piece of cake”).
We also offer four IP-free vectors with different features, all designed to improve protein expression and stability.
GTP has expertise with other expression systems
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