Capabilities

Lactococcus lactis expression system

GTP's proprietary bacterial expression system LactXpress uses the Lactococcus lactis bacteria. LactXpress is an alternative and innovative expression system for the production of challenging recombinant proteins.

LactXpress provides an innovative alternative for the production of high-added-value and challenging proteins

The LactXpress bacterial expression system for easy purification and efficient scale-up

L. lactis

GTP's proprietary LactXpress expression system offers several advantages compared to other bacterial systems. One of the main advantages of Lactococcus lactis is that the recombinant protein is efficiently secreted into the culture medium, with few endogenous proteins, resulting in a simplified purification process.

Since L. lactis does not produce endotoxins, this represents a great advantage when compared to E. coli. As the system offers a very simple metabolism and rapid growth without aeration, L. lactis is easy to cultivate and the production volume can be readily scaled up from a few millilitres to thousands of litres in just one step.

Our LactXpress system includes both inducible (Patent file n°FR0210805) and constitutive promoters (Patent FR 0954785). With our protease-free strain, L. lactis htrA (Patent file n°FR 9816462) is devoid of any surface protease, which allows us to improve expression yield and recombinant protein stability.

Feasibility services & licensing opportunities to evaluate LactXpress for your protein

To evaluate the LactXpress system for your specific protein, we offer custom feasibility study services, which include vector construction, expression trials in mini-reactors with wild-type or HtrA- strains, and the delivery of protein samples.

Should you prefer to try the system out by yourself, LactXpress is available for licensing. For research projects, we offer an easy-to-use research license. If your application is industrial, exclusive or non-exclusive commercial licenses can be obtained. Do not hesitate to contact us for more information or to discuss the terms of the license agreement.