P. pastoris, a cost-effective expression system
Pichia pastoris is one of the most extensively used systems for recombinant protein expression. The expiry of key patents, as well as emerging new technologies, open the door for more types of proteins and applications, such as new antibody scaffolds.
Pichia's greatest advantage results from its high heterologous protein secretion capacity, which, combined with low levels of endogenous protein secretion, leads to simplified downstream processes. In addition, Pichia pastoris can be grown to high cell densities, resulting in high protein yields per litre of culture, which makes it a highly cost-efficient eukaryotic system.
The GTP team has successfully used Pichia system to express enzymes, cytokines, antibody-derived fragments…