Microbial upstream process development

GTP provides expert upstream process development services for microbial expression systems.

Our scientists draw from their vast experience with a large number of processes to successfully transfer your project to manufacturing and help you overcome your microbial expression challenge.

Customised & efficient microbial expression systems to fit your project need

GTP's scientists have supported over 20 microbial process development programs, delivering either processes for decagram batches such as for preclinical studies, or a robust and scalable process that can be successfully transferred to a GMP facility for manufacturing.

Whether you're looking for E. coli or P. pastoris systems, we always focus on developing high-expressing strains. We also identify optimal expression conditions for high titer and determine critical quality attributes to ensure process robustness and scalability.

We always keep the nature of your protein and your IP constraints in mind to suggest the most appropriate expression platform. For E. coli, the best option is generally to use our optimised IP-free vectors, which are designed to improve protein expression and stability. 

For very challenging proteins, when traditional E. coli systems fail, we evaluate innovative E. coli strains through our partnerships with research platforms and technology companies.

GTP is an agile organisation and offers the flexibility to meet your timelines through fast-track process development.

Robust, scalable & GMP-ready upstream processes

GTP offers a comprehensive set of process development services to deliver GMP ready upstream processes:

  • Strain Development.
  • Selection of strain and cell bank generation (RCB & MCB)
  • Media & feed development and optimisation.
  • Development of batch, fed-batch or perfusion processes.
  • Identification of critical process parameters.
  • Optimisation of existing processes.
  • Support for transfer to manufacturing.

State-of-the-art facilities

Our fermentation facilities offer the appropriate equipment to cover every stage of the development process: from 2 to 5L glass fermenters for process optimisation, to 50L stainless steel fermenters for pilot production. The methods and platforms used are among the standards of most CMOs, for a cost-effective, timely and seamless transfer to the cGMP manufacturing suite of your choice.

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