Analytical development

Expert analytical services to support your protein process development

Enzymes, antigens, antibodies and peptides have no secrets for us: we have worked on over 800 diverse recombinant protein projects to vouch for our expertise. We have a strong expertise in developing a relevant analytical package to support upstream and downstream process development for each project.

Analytical methods 2

Expert analytic services to help your protein project move forward

GTP has over 19 years of experience in recombinant protein projects at every stage of development. We master an extensive range of analytical methods to expertly support your project.

Our expertise includes in-process testing, characterization of product critical attributes, formulation and stability studies. To fit your project's specific needs, we draw from our experience and select the most appropriate analytical techniques and methods.

Our service include analytical methods transfer, development, qualification to support the development of upstream and dowstream processes.

Electrophoretic techniques

  • SDS-PAGE
  • Capillary electrophoresis
  • IEF
  • Western-blot

HPLC

  • Size exclusion
  • Reverse phase
  • Ion exchange
  • Affinity

Process residuals

  • HCP determination by ELISA
  • Endotoxins quantification by LAL
  • DNA quantification by qPCR
  • Microbiological contamination (Bioburden)

Potency assays

  • Enzymatic activity
  • Binding assays via Elisa and BioLayer Interferometry (BLItz®)

Mass spectrometry

  • Intact mass
  • Peptide mapping
  • Post translational modifications
  • Glycan profile

Biophysical characterisation

  • DLS
  • MALS
  • Thermal shift assay (Thermofluor®)

 

Reliable and comprehensive analytical services to support all your recombinant protein production projects