Large scale production
GTP has a solid experience of industrial recombinant protein production acquired through 10 years partnership with clients in various industries.
Our track record includes:
- The production of candidate therapeutic proteins for preclinical investigation
- The recurrent production of batches of antigens for use in diagnostic kits
GTP provides fully characterized proteins up to 100 grams scale.
Our capabilities range from mg up to hundred grams of recombinant protein
Microbial cell culture:
- organisms: E. coli, L. lactis, S. cerevisiae, P. pastoris
- scale: 20L, 50L, 300L fermentors
Animal cell culture:
- organisms: mammalian cells (CHO derived cell lines, BHK, HEK293 derived cell lines, SP2...), insect cells (Sf9, High 5, S2)
- scale: 5L, 10L, 25L, 50L (Wave bioreactors)
Purification:
- scale: quantities up to 100 grams
- systems: 30L/h gradient chromatography skid BioProcess (GE Healthcare) – Cross-flow filtration skids up to 68L/min (membranes and/or hollow fiber, Millipore and GE Healthcare)
QC :
| -SDS-PAGE -Capillary electrophoresis -Western-Blot -HPLC (SE and RP) |
-Bradford -BCA -Elisa -Activity test |
-Mass spectrometry -N-terminal sequencing -Peptide mapping |
-Residual DNA -Endotoxin level -Host cell proteins |