Analytics
Whether the protein is to be used for research, in preclinical animal experiments or as the active ingredient of a marketed product, GTP will perform an extensive set of in-process controls and end-product analysis.
Methods used at GTP to characterise proteins comprise:
Protein identification methods:
- SDS-PAGE
- Capillary electrophoresis
- Western-Blot
- HPLC
- Mass spectrometry
- N-terminal sequencing
- Peptide mapping
Methods to determine the purity and detect contaminants:
- Densitometric analysis on SDS-PAGE
- HPLC
- Residual DNA
- Endotoxin level
- Host cell proteins
Protein quantification methods:
- HPLC
- Bradford
- BCA
- Elisa
- Activity test
Other methods available:
- DLS