tailor-made services:  from gene cloning to purified protein

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From Gene To Protein

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Our multidisciplinary team expertise covers all techniques involved in a recombinant protein production project.


 Sub-cloning
Vector construction
Gene synthesis
   Multiple expression systems
Capacity ranging from 500 up to 250L
Molecular Biology Expression Purification
Strategy  Downstream  Standard or « structural quality » purification
Protocol development
 Theoretical studies    Quality controls


A « typical » project from gene to protein

We structure our contract projects in phases with built-in milestones and regularly keep you updated on the status of your projects. A the end of each phase, we request for your validation before proceeding to the next step.     
                                                                                                                                                                                              
Material needed

  • Plasmid containing sequence of interest
  • Proteic or nucleotidic sequence
Feasability study
  • Expression assay
  • Expression vector construction
  • Production assays
  • Production yield evaluation
  • Results analysis
Report delivery => Customer validation GO / NO-GO
  • Test batch production for customer validation
  • Production in the best conditions specified after the expression assays
  • Purification
  • Quality controls

Report and test batch delivery => Customer validation GO / NO-GO

Batch production
  • Production and purification of a batch identical to test batch
  • Quality controls
Deliverables
  • Purifed protein
  • Data sheet
  • Detailed report

 

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