The quality of services provided by GTP Technology is closely related to the maintenance of a significant R&D activity. Based on its technological platform for protein production and protein engineering, GTP Technology is running R&D programs to develop innovative biotechnological tools.
L. Lactis program
An innovative system designed to manufacture pharmaceutical grade proteins.
L.lactis program is GTP Technology principal axis of research.
This research, initiated in 2003 (license on two INRA patents), led to the development of an innovative expression system that offers a large number of significant advantages compared to classical expression systems.
GTP Technology now proposes the L.lactis system for therapeutic proteins production : research contracts and out licensing
In parallel, we are continuously improving the L.lactis system, in collaboration with INRA (Jouy-en-Josas) and INSA (Toulouse). This program is partly subsidized by ANVAR.
Publications :
Llull D. and Poquet I. (2004) New expression system tightly controlled by zinc availability in Lactococcus lactis. Appl Environ Microbiol 70, 5398-406.
Poquet I., Saint V., Seznec E., Simoes N., Bolotin A. and Gruss A. (2000) HtrA is the unique surface housekeeping protease in Lactococcus lactis and is required for natural protein processing. Mol Microbiol 35, 1042-51.
Le Loir Y., Azevedo V., Oliveira S. C., Freitas D. A., Miyoshi A., Bermudez-Humaran L. G., Nouaille S., Ribeiro L. A., Leclercq S., Gabriel J. E., Guimaraes V. D., Oliveira M. N., Charlier C., Gautier M. and Langella P. (2005) Protein secretion in Lactococcus lactis : an efficient way to increase the overall heterologous protein production. Microb Cell Fact 4, 2
Le Loir Y., Nouaille S., Commissaire J., Bretigny L., Gruss A. and Langella P. (2001) Signal peptide and propeptide optimization for heterologous protein secretion in Lactococcus lactis. Appl Environ Microbiol 67, 4119-27.
Miyoshi A., Poquet I., Azevedo V., Commissaire J., Bermudez-Humaran L., Domakova E., Le Loir Y., Oliveira S. C., Gruss A. and Langella P. (2002) Controlled production of stable heterologous proteins in Lactococcus lactis. Appl Environ Microbiol 68, 3141-6.
Nouaille S., Ribeiro L. A., Miyoshi A., Pontes D., Le Loir Y., Oliveira S. C., Langella P. and Azevedo V. (2003) Heterologous protein production and delivery systems for Lactococcus lactis. Genet Mol Res 2, 102-11.
Poquet I., Ehrlich S. D. and Gruss A. (1998) An export-specific reporter designed for gram-positive bacteria: application to Lactococcus lactis. J Bacteriol 180, 1904-12.
3D Repertoire
A multidisciplinary approach to determine the structures of protein complexes in a model organism.
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3D-Repertoire is an integrated project funded by the European Commission under Framework 6. It brings together scientists from 14 institutions and private companies, in seven countries across Europe, with the aim to resolve structures for all amenable protein complexes in a model organism.
This program will lead to the development of new molecular tools allowing production of 2nd generation therapeutic targets (multi-proteic complexes).
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We live in an era when the entire genetic code of many organisms has been established. However, to make full use of this information, we need to bring it together with the knowledge of what the products of all these genes, the proteins, are doing.
These proteins rarely act alone: they typically interact with other macromolecules to perform particular cellular tasks. So, the discovery and analysis of cellular protein complexes will provide information not only on complex functions but also provide much valuable data including insights into protein-protein interaction interfaces for interacting components in many important cellular systems.
This elucidation of three-dimensional structures for protein complexes will open new avenues to unravel the molecular pathology and physiology of human diseases, leading to rational, target-oriented therapeutic approaches.
GTP Technology mission is dedicated to the development of innovative strategies for production and purification of protein complexes.
Novtech
Novel technology for controlling wine production and quality
Production of high quality food and beverage products requires a permanent monitoring of the production process and the quality of the final product, presently assessed by using highly sophisticated and expensive off line analytical methods, not affordable for SMEs or individual producers.
This aspect is vital for the wine industry, since this industry is dominated by a large number of small producers. An adequate control for this production requires continuous, simultaneous and selective monitoring of several key compounds determining the quality and special flavour of a particular wine. Such detectors are non-existing on the market.
The multidisciplinary research project Novtech targets the development of a highly innovative novel technology for the control of wine production and quality, integrating sampling and detection, and allowing continuous sampling and simultaneous monitoring of several analytes. The sensors/sensor arrays to be developed are based both on commercially available and novel engineered enzymes- and/or antibodies.
GTP is working on the engineering of biomolecules, designed for the selective detection of wine key analytes.
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