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> If you identify and clone numerous genes,
> If you need to produce a protein but you do not have the gene,
> If you want a customized vector,
GTP Technology Gene Work platform can help you.
By entrusting us with your projects of cloning, gene synthesis and vector construction :
- You will save time to concentrate on the crucial points of your research
- You will benefit from our expertise.
OUR STRENGTHS
Short turnaround times. Our Gene Work platform is dedicated to efficient cloning and vector construction.
Flexibility. Your can choose the acceptor vector and the restriction sites adapted to your application.
Quality. We guarantee a 100% match between synthetized or sub-cloned sequences and requested sequences.
OBTAINING YOUR GENE OF INTEREST
You don't have the gene of interest: we help you to determine the best strategy to get it.
CLONING
We can retrieve your gene of interest from any source (genomic DNA, tissues, cells, etc.) and clone it into the vector of your choice.
We will first perform a bibliographic / bioinformatic study to define the best strategy to get your gene.
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Service overview
Gene isolation (RT-PCR, PCR, …)
Subcloning into acceptor vector
Recombinant clone amplification and purification
Verification by sequencing
You will receive
Purified plasmidic DNA
Report including sequence datas and construction map in electronic form
Delivery time
4 - 6 weeks
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GENE SYNTEHSIS / SEQUENCE OPTIMIZATION
> Your gene expression source is difficult to access or you do not know in which tissue the gene is expressed,
> You want to enhance the expression of your protein by codon optimization,
> You want to design your own sequence / to create new intellectual property,
> You need to modify your gene for structure-function studies...
We can synthetise your requested genes "de novo".
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Service overview
Sequence design: codon or sequence optimization if needed
Gene synthesis
Subcloning into standard GTP vector or any specified vector
Sequence verification by double strand sequencing
You will receive
Purified plasmidic DNA
Report including sequence and construction map
Delivery time
4 - 6 weeks
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VECTOR CONSTRUCTION
We can perform your clonings into GTP standard vectors or any specified alternative vector and customize your vector as requested.
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Service overview
Counseling on vector design
“Standard” subcloning into the vector of your choice or vector customization (tag addition, promoter exchange, tag removal sequence insertion ,etc…)
Recombinant clone amplification and purification
Verification by sequencing
You will receive
Purified plasmidic DNA
Report including sequence and construction map
Delivery time
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