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Q-PCR is a fast, accurate and reliable means to measure quantitatively the distribution and expression of target DNA or RNA. This technology is currently used for numerous applications :
> Gene expression profiling,
> Analysis of biological response to drug compounds,
> Microarrays results validation,
> Diagnostic : detection of pathogens, virus quantification…
GTP Technology complements its services in now providing solutions for development and optimization of Real-Time PCR assays on Cepheid SmartCycler.
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WHY USE OUR SERVICES ?
Customization. Our molecular biologists can design a custom assay for your specific application.
Time saving. In entrusting us with your assay development, you will save time to concentrate on the crucial points of your research.
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Our offer
custom real-time PCR assay development for Smart Cycler
> You need an assay for a diagnostic application : you simply know the disease or the microorganism, you want to detect a specific RNA.
> You want to analyse the expression profile of a specific gene : drug or cosmetic compounds response analysis,...
But there is no existing assay available for your application..
GTP Technology can develop your own custom assay : we perform all steps from the strategy choice to the protocol testing.
Adaptation of an existing Real-Time PCR assay
> You want to convert your classical PCR protocol to a Real-Time PCR protocol : we can design your new probe.
> You want to tranfer your protocol from a competitor system to SmartCycler : we can help to change the fluorochrome.
> You need to change the chemistry of your probe (from Taqman to Scorpion to gain more specificity…). 
GTP Technology improves your existing protocol to help you with these issues.
- Strategy choice :
- Identification of the optimal target sequence,...
- Assay development :
- Primers and probe design
- Optimization of reaction conditions
- Standards choice and calibration
- Protocol optimization :
- Optimization of sensivity, specificity and reproductibility
- Protocol validation :
- Sample analysis :
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